Which practice is recommended to ensure accuracy when performing tests on serum samples?

Which practice is recommended to ensure accuracy when performing tests on serum samples?

• A. Run each sample once
• B. Run each sample at least three times
• C. Run each sample twice
• D. Run only as needed

Answer: (B)

Repeating tests in multiple replicates helps distinguish the true sample value from random errors that can creep in during handling, pipetting, or instrument variation. If you run a serum test only once, a small mistake or anomaly can skew the result. Running it twice improves confidence, but still leaves room for an outlier to go unnoticed. Performing the test three or more times allows you to average the results, giving a value that better reflects the actual serum concentration, and it provides a sense of precision by showing how close the results are to each other. It also helps identify any outliers that should be repeated or flagged according to quality control rules. In serum testing, using triplicates is a standard approach to achieve more reliable and accurate results, rather than relying on a single measurement or ad-hoc repeats.

Repeating tests in multiple replicates helps distinguish the true sample value from random errors that can creep in during handling, pipetting, or instrument variation. If you run a serum test only once, a small mistake or anomaly can skew the result. Running it twice improves confidence, but still leaves room for an outlier to go unnoticed. Performing the test three or more times allows you to average the results, giving a value that better reflects the actual serum concentration, and it provides a sense of precision by showing how close the results are to each other. It also helps identify any outliers that should be repeated or flagged according to quality control rules. In serum testing, using triplicates is a standard approach to achieve more reliable and accurate results, rather than relying on a single measurement or ad-hoc repeats.