What is tested in a minor crossmatch?

What is tested in a minor crossmatch?

• A. T-cell production
• B. Recipient serum against donor erythrocytes
• C. Recipient erythrocytes against donor serum
• D. Recipient urine against donor erythrocytes

Answer: (C)

In a minor crossmatch you’re looking for antibodies present in the donor’s plasma that could attack the recipient’s red blood cells. Practically, this means mixing donor serum with recipient erythrocytes and watching for an reaction. If the donor’s antibodies target the recipient’s RBC antigens, agglutination or hemolysis would occur, signaling incompatibility from the donor’s antibodies. That’s why the correct approach is recipient erythrocytes tested against donor serum. The other concept (recipient serum against donor erythrocytes) would be a major crossmatch, aimed at detecting the recipient’s antibodies against donor cells. T-cell production and urine interactions aren’t part of crossmatching.

In a minor crossmatch you’re looking for antibodies present in the donor’s plasma that could attack the recipient’s red blood cells. Practically, this means mixing donor serum with recipient erythrocytes and watching for an reaction. If the donor’s antibodies target the recipient’s RBC antigens, agglutination or hemolysis would occur, signaling incompatibility from the donor’s antibodies.

That’s why the correct approach is recipient erythrocytes tested against donor serum. The other concept (recipient serum against donor erythrocytes) would be a major crossmatch, aimed at detecting the recipient’s antibodies against donor cells. T-cell production and urine interactions aren’t part of crossmatching.